DOI:

10.37988/1811-153X_2024_1_66

Elimination of critical jawbone defects based on the use of xenogeneic collagen membranes and amorphous hydroxyapatite in an experiment

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Authors

  • E.Yu. Diachkova 1, PhD in Medical Sciences, associate professor of the Surgical dentistry Department
    ORCID ID: 0000-0003-4388-8911
  • B.P. Ershov 1, 4th year student, laboratory assistant at the Laboratory of Regenerative Veterinary Medicine
    ORCID ID: 0000-0002-3879-2424
  • I.A. Demyanenko 2, PhD in Biology, researcher at the Laboratory of effectors and mediators of immunity
    ORCID ID: 0000-0002-8238-7100
  • N.V. Kalmykova 2, PhD in Biology, researcher at the Laboratory of effectors and mediators of immunity
    ORCID ID: 0000-0001-9075-3361
  • A.L. Fayzullin 1, PhD in Medical Sciences, head of the Digital microscopic analysis Lab
    ORCID ID: 0000-0003-4137-8993
  • A.A. Marakhovskaya 1, 6th year student
    ORCID ID: 0000-0002-5839-0000
  • M.M. Petukhova 1, 4th year student, research intern of the Surgical dentistry Department
    ORCID ID: 0000-0002-2071-4487
  • 1 Sechenov University, 119991, Moscow, Russia
  • 2 N.F. Gamaleya Research Institute of Epidemiology and Microbiology, 123098, Moscow, Russia

Abstract

Elimination of jaw defects of various etiologies remains a pressing issue for many years. The aim of the work was to analyze osteogenesis in the area of critical defects in the jaws of laboratory animals using various combinations of xenogeneic dental materials Matriflex (Biopharmaholding, Moscow, Russia) in the form of hydroxyapatite crumbs and two types of collagen membranes.
Materials and methods.
A study was conducted on 6 Chinchilla rabbits. During the experiment, critical defects were created intraoperatively in all animals in the lower jaw area with their subsequent filling with osteoplastic materials in 3 combinations (hydroxyapatite, hydroxyapatite and collagen membrane no. 1, hydroxyapatite and collagen membrane no. 2), as well as one defect each without filling with material as a control area for natural regeneration. In the postoperative period, local edema and hyperemia were count according to the scoring system. The observation period was 3 months, followed by removal of animals from the experiment and collection of biomaterial for microfocus computed tomography (CT) and histological examination.
Results.
According to the control postoperative examinations of animals, no statistically significant differences were obtained in the severity of edema and tissue hyperemia in the area of defects treated with different methods. According to the results of microfocus CT, the healing of defects from 80 to 100% was determined in the area of application of hydroxyapatite and collagen membranes, especially compared to defects without filling with materials. Histological examination confirmed the CT results — regeneration was more pronounced in the area of implantation of materials.
Conclusion.
The use of xenogeneic materials Matriflex in the form of membranes and hydroxyapatite makes it possible to almost completely eliminate critical jaw defects in experiments and can be recommended for clinical practice.

Key words:

osteoplastic material, defect, jaw, hydroxyapatite, collagen, experiment

For Citation

[1]
Diachkova E.Yu., Ershov B.P., Demyanenko I.A., Kalmykova N.V., Fayzullin A.L., Marakhovskaya A.A., Petukhova M.M. Elimination of critical jawbone defects based on the use of xenogeneic collagen membranes and amorphous hydroxyapatite in an experiment. Clinical Dentistry (Russia).  2024; 27 (1): 66—74. DOI: 10.37988/1811-153X_2024_1_66

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Received

September 7, 2023

Accepted

January 17, 2024

Published on

March 21, 2024