DOI:

10.37988/1811-153X_2025_2_50

The effect of platelet-rich fibrin (PRF) on orthodontic tooth movement: A split-mouth randomized controlled trial

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Authors

  • T.P. Chau Minh 1, MSc., DDS, faculty Odonto-stomatology
  • N.L. Lam 1, PhD, DDS, head of Department of Pediatrics Dentistry and Orthodontics, vice dean of faculty of Odonto-Stomatology
    ORCID: 0000-0003-1393-0314
  • V.L. Khanh Phuong 1, DDS, faculty Odonto-stomatology
    ORCID: 0000-0001-5399-1811
  • 1 Can Tho University of Medicine and Pharmacy, 900000, Can Tho, Vietnam

Abstract

Objective — to evaluate the effectiveness of injectable platelet-rich fibrin (i-PRF) in accelerating orthodontic tooth movement (OTM) and reducing treatment duration.
Materials and methods.
This study used a split-mouth randomized clinical trial with 31 patients (14—31 years old, 9 males and 22 females) with Angle Class I malocclusion indicated bilateral maxillary first premolar extraction with a double-blind design. The i-PRF was obtained from each patient‘s blood and then injected into the buccal and palatal areas of the extraction sites right before the canines were retracted. The i-PRF was used on the left side, and physiological saline was used on the right side of the patients with even numbers. On the other hand, the i-PRF was used on the right side for patients with an odd number, whereas physiological saline was used on the left side. After the leveling and alignment phases, the canines were retracted with 150 g of force. Digital models were obtained at five time points: before tooth extraction, in the fourth, eighth, twelfth, and sixteenth week from the beginning of distalization.
Results.
In the current study, the rate of canine tooth movement was higher in the study group (PRF injection) than in the control group at all time points (p<0.001). PRF injection increased orthodontic tooth movement during the early stage (first 2 months). The average acceleration of canine movement in the PRF injection group was 1.21 times. There was no statistically significant difference between the male and female genders.
Conclusion.
i-PRF can be an effective method for accelerating orthodontic movement with minimal invasion, safety, and being autogenous.

Key words:

injectable platelet-rich fibrin, i-PRF, orthodontic tooth movement, OTM, canine retraction

For Citation

[1]
Chau Minh T.P., Lam N.L., Khanh Phuong V.L. The effect of platelet-rich fibrin (PRF) on orthodontic tooth movement: A split-mouth randomized controlled trial. Clinical Dentistry (Russia).  2025; 28 (2): 50—56. DOI: 10.37988/1811-153X_2025_2_50

References

  1. Zeitounlouian T.S., Zeno K.G., Brad B.A., Haddad R.A. Three-dimensional evaluation of the effects of injectable platelet rich fibrin (i-PRF) on alveolar bone and root length during orthodontic treatment: a randomized split mouth trial. BMC Oral Health. 2021; 21 (1): 92. PMID: 33653326
  2. Zeitounlouian T., Haddad R., Brad B., Ballouk M.A., Fudalej P. Does the application of autologous injectable Platelet-Rich Fibrin (i-PRF) affect the patient‘s daily performance during the retraction of upper canines? A single-centre randomized split-mouth controlled trial. BMC Oral Health. 2023; 23 (1): 872. PMID: 37978474
  3. Aboalnaga A.A., Salah Fayed M.M., El-Ashmawi N.A., Soliman S.A. Effect of micro-osteoperforation on the rate of canine retraction: a split-mouth randomized controlled trial. Prog Orthod. 2019; 20 (1): 21. PMID: 31155698
  4. Gurbax S., Raahat V.S., Roopsirat K., Devinder P.S. Accelerated orthodontic tooth movement: A review. Modern Research in Dentistry. 2017; 1 (2): 35—38. DOI: 10.31031/MRD.2017.01.000508
  5. Xue H., Zheng J., Cui Z., Bai X., Li G., Zhang C., He S., Li W., Lajud S.A., Duan Y., Zhou H. Low-intensity pulsed ultrasound accelerates tooth movement via activation of the BMP-2 signaling pathway. PLoS One. 2013; 8 (7): e68926. PMID: 23894376
  6. Chang J., Chen P.J., Dutra E.H., Nanda R., Yadav S. The effect of the extent of surgical insult on orthodontic tooth movement. Eur J Orthod. 2019; 41 (6): 601—608. PMID: 30828725
  7. Agrawal D.R., Jaiswal P.G. Injectable Platelet Rich Fibrin (i-PRF): A Gem in Dentistry. International Journal of Current Research and Review. 2020; 21: 25—30 (In). DOI: 10.31782/IJCRR.2020.122116
  8. Che Y., Li P., Tian L., Wang M., Xiong R., Lei X., Li X., Ge Zh. The research progress of platelet-rich fibrin applications in the orthodontic treatment. Advances in Computer Science Research. 2017; 59: 524—529. DOI: 10.2991/emcm-16.2017.99
  9. Choukroun J., Ghanaati S. Reduction of relative centrifugation force within injectable platelet-rich-fibrin (PRF) concentrates advances patients‘ own inflammatory cells, platelets and growth factors: the first introduction to the low speed centrifugation concept. Eur J Trauma Emerg Surg. 2018; 44 (1): 87—95. PMID: 28283682
  10. Francisco I., Fernandes M.H., Vale F. Platelet-Rich Fibrin in Bone Regenerative Strategies in Orthodontics: A Systematic Review. Materials (Basel). 2020; 13 (8): 1866. PMID: 32316144
  11. Wang X., Zhang Y., Choukroun J., Ghanaati S., Miron R.J. Effects of an injectable platelet-rich fibrin on osteoblast behavior and bone tissue formation in comparison to platelet-rich plasma. Platelets. 2018; 29 (1): 48—55. PMID: 28351189
  12. Ozsagir Z.B., Saglam E., Sen Yilmaz B., Choukroun J., Tunali M. Injectable platelet-rich fibrin and microneedling for gingival augmentation in thin periodontal phenotype: A randomized controlled clinical trial. J Clin Periodontol. 2020; 47 (4): 489—499. PMID: 31912532
  13. Saglam E., Ozsagir Z.B., Unver T., Alinca S.B., Toprak A., Tunali M. Efficacy of injectable platelet-rich fibrin in the erosive oral lichen planus: a split-mouth, randomized, controlled clinical trial. J Appl Oral Sci. 2021; 29: e20210180. PMID: 34614123
  14. Pham C.T.M., Le L.N., Phuong Le K.V., Truong K.N. Is the injectable platelet-rich fibrin (i-PRF) an effective method for root length and alveolar bone in maxillary canine retraction? A split-mouth randomized controlled trial. The Open Dentistry Journal. 2025; 19: e18742106363883. DOI: 10.2174/0118742106363883250203060336
  15. Barsoum H.A., ElSayed H.S., El Sharaby F.A., Palomo J.M., Mostafa Y.A. Comprehensive comparison of canine retraction using NiTi closed coil springs vs elastomeric chains. Angle Orthod. 2021; 91 (4): 441—448. PMID: 34181721
  16. Reyes Pacheco A.A., Collins J.R., Contreras N., Lantigua A., Pithon M.M., Tanaka O.M. Distalization rate of maxillary canines in an alveolus filled with leukocyte-platelet-rich fibrin in adults: A randomized controlled clinical split-mouth trial. Am J Orthod Dentofacial Orthop. 2020; 158 (2): 182—191. PMID: 32591274
  17. El-Timamy A., El Sharaby F., Eid F., El Dakroury A., Mostafa Y., Shaker O. Effect of platelet-rich plasma on the rate of orthodontic tooth movement. Angle Orthod. 2020; 90 (3): 354—361. PMID: 33378433
  18. Erdur E.A., Karakaslı K., Oncu E., Ozturk B., Hakkı S. Effect of injectable platelet-rich fibrin (i-PRF) on the rate of tooth movement. Angle Orthod. 2021; 91 (3): 285—292. PMID: 33459765
  19. Krishnan V., Davidovitch Z. Cellular, molecular, and tissue-level reactions to orthodontic force. Am J Orthod Dentofacial Orthop. 2006; 129 (4): 469.e1—32. PMID: 16627171
  20. Koyanagi M., Fujioka-Kobayashi M., Yoneyama Y., Inada R., Satomi T. Regenerative Potential of Solid Bone Marrow Aspirate Concentrate Compared with Platelet-Rich Fibrin. Tissue Eng Part A. 2022; 28 (17—18): 749—759. PMID: 35357952
  21. Miron R.J., Fujioka-Kobayashi M., Hernandez M., Kandalam U., Zhang Y., Ghanaati S., Choukroun J. Injectable platelet rich fibrin (i-PRF): opportunities in regenerative dentistry?Clin Oral Investig. 2017; 21 (8): 2619—2627. PMID: 28154995
  22. Kobayashi E., Flückiger L., Fujioka-Kobayashi M., Sawada K., Sculean A., Schaller B., Miron R.J. Comparative release of growth factors from PRP, PRF, and advanced-PRF. Clin Oral Investig. 2016; 20 (9): 2353—2360. PMID: 26809431
  23. Shah R., Gowda T.M., Thomas R., Kumar T., Mehta D.S. Biological activation of bone grafts using injectable platelet-rich fibrin. J Prosthet Dent. 2019; 121 (3): 391—393. PMID: 30409723
  24. Farshidfar N., Jafarpour D., Firoozi P., Sahmeddini S., Hamedani S., de Souza R.F., Tayebi L. The application of injectable platelet-rich fibrin in regenerative dentistry: A systematic scoping review of In vitro and In vivo studies. Jpn Dent Sci Rev. 2022; 58: 89—123. PMID: 35368368
  25. Seifi M., Badiee M.R., Abdolazimi Z., Amdjadi P. Effect of basic fibroblast growth factor on orthodontic tooth movement in rats. Cell J. 2013; 15 (3): 230—7. PMID: 24027664
  26. Tehranchi A., Behnia H., Pourdanesh F., Behnia P., Pinto N., Younessian F. The effect of autologous leukocyte platelet rich fibrin on the rate of orthodontic tooth movement: A prospective randomized clinical trial. Eur J Dent. 2018; 12 (3): 350—357. PMID: 30147398
  27. Mheissen S., Daraqel B., Alzoubi E.E., Khan H. Effectiveness of platelet-rich concentrates on the rate of orthodontic tooth movement: a systematic review and meta-analysis. Eur J Orthod. 2023; 45 (2): 196—207. PMID: 36056906
  28. Sharan J., Shivakumar I., Shivakumar A., Kamal V.K., Chaudhari P.K., Challasany S., Marya A. Does the use of platelet-rich fibrin enhance the rate of orthodontic tooth movement? A systematic review and meta-analysis. J Oral Biol Craniofac Res. 2024; 14 (2): 192—200. PMID: 38434677
  29. Karakasli K., Erdur E.A. The effect of platelet-rich fibrin (PRF) on maxillary incisor retraction rate. Angle Orthod. 2021; 91 (2): 213—219. PMID: 33347530
  30. Wallace H.A., Basehore B.M., Zito P.M. Wound healing phases. StatPearls, 2025. PMID: 29262065

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Received

December 7, 2024

Accepted

April 1, 2025

Published on

July 5, 2025