DOI:

10.37988/1811-153X_2023_4_64

Microbial adhesion to samples of complete removable dentures of polymethylmethacrylate-polyethyleneterephthalate composition

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Authors

  • E.A. Chizhmakov 1, assistant at the Propaedeutics and prosthodontics technology Department
    ORCID: 0000-0003-1313-3307
  • T.V. Tsareva 1, PhD in Medical Sciences, associate professor of the Microbiology, virology, immunology Department
    ORCID: 0000-0001-9571-0520
  • M.S. Podporin 1, PhD in Medical Sciences, researcher at the Molecular biology research Laboratory of the Medico-dental research Institute
    ORCID: 0000-0001-6785-0016
  • E.V. Ippolitov 1, PhD in Medical Sciences, professor of the Microbiology, virology, immunology Department
    ORCID: 0000-0003-1737-0887
  • I.V. Tlupov 2, assistant at the Institute of Dentistry and maxillofacial surgery
    ORCID: 0009-0003-4474-5434
  • Z.E. Balagova 2, assistant at the Institute of Dentistry and maxillofacial surgery
    ORCID: 0000-0001-5157-9006
  • M.V. Timoshchenko 1, PhD in Medical Sciences, assistant professor of the Digital dentistry Department
    ORCID: 0000-0002-6949-9351
  • A.S. Arutyunov 1, PhD in Medical Sciences, full professor of the Propaedeutics and prosthodontics technology Department
    ORCID: 0000-0002-2655-6707
  • 1 Moscow State University of Medicine and Dentistry, 127473, Moscow, Russia
  • 2 Kabardino-Balkarian State University, 360004, Nalchik, Russia

Abstract

The processes of primary adhesion to structural materials determine the initial stage of formation of aggressive microbial biofilms, which, in turn, play an extremely important role in the development of infectious complications. The aim of the study is to evaluate the adhesive activity of various representatives of oral microbiota to samples of dental materials made of polyethylene terephthalate (PET) and polymethyl methacrylate (PMMA) taking into account their hydrophobic properties, as well as to assess the relationship between bacteria-substrate pairs in the combination of PET and PMMA.
Materials and methods.
Based on a significance level of 0.05 and power of 0.8 the study produced 16 samples of each material (n=80): PET; PMMA (baseline); PMMA (garnished); combination of PET and PMMA materials (baseline); combination of PET and PMMA materials (garnished). The primary adhesion index (AI) was calculated and compared to samples of test strains of S. sanguinis, A. naeslundii, N. sicca, P. micra, P. gingivalis, F. periodonticum, C. albicans and C. krusei isolated from periodontal pockets of patients with chronic periodontitis, and the degree of hydrophobicity of these microbes was calculated.
Results.
Adhesion of representatives of normobiota with hydrophobic properties (A. naeslundii) to PET was minimal (AI=0.27±0.03, p<0.05). The lowest AI values were determined for strains with amphiphilic and hydrophilic properties to PMMA (p<0.05). Optimal AI (0.52±0.03, p<0.05) was found for the combination of PET and PMMA (garnished).
Conclusions.
The results obtained in the presented study allow us to conclude about the role of hydrophobicity of representatives of the oral microbiome, as well as the chemical nature of structural materials and their combinations as factors influencing the primary adhesion of microorganisms.

Key words:

denture bases, denture, complete, immediate, polyethylene terephthalates, polymethyl methacrylate, tooth, artificial

For Citation

[1]
Chizhmakov E.A., Tsareva T.V., Podporin M.S., Ippolitov E.V., Tlupov I.V., Balagova Z.E., Timoshchenko M.V., Arutyunov A.S. Microbial adhesion to samples of complete removable dentures of polymethylmethacrylate-polyethyleneterephthalate composition. Clinical Dentistry (Russia).  2024; 26 (4): 64—70. DOI: 10.37988/1811-153X_2023_4_64

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Received

August 14, 2023

Accepted

November 2, 2023

Published on

January 16, 2024