DOI:

10.37988/1811-153X_2026_1_153

Evaluation of the osteogenic potential of the surface of dental implants coated with recombinant BMP-2 in an in vivo experiment

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Authors

  • A.G. Stepanov 1, Doctor of Science in Medicine, professor of the Institute of Digital Dentistry
    ORCID: 0000-0002-6543-0998
  • S.V. Apresyan 1, Doctor of Science in Medicine, professor, director of the Institute of Digital Dentistry
    ORCID: 0000-0002-3281-707X
  • E.G. Nacharyan 1, dentist, PhD candidate at the Institute of Digital Dentistry
    ORCID: 0009-0005-0081-915X
  • M.V. Kopylov 1, PhD in Medical Sciences, assistant at the Institute of Digital Dentistry
    ORCID: 0000-0001-8567-2225
  • V.S. Levina 1, 4th year student
    ORCID: 0009-0002-3290-3805
  • 1 RUDN University, 117198, Moscow, Russia

Abstract

Objective: To evaluate the osteogenic potential of dental implants coated with recombinant bone morphogenetic protein-2 (BMP-2) in an in vivo experiment.
Materials and methods.
An experimental dental implantation model was used. Control implants had no bioactive coating, while experimental implants were coated with BMP-2. Osseointegration was assessed at 3- and 5-months using CBCT, histological, morphometric, and immunohistochemical analyses (SPARC/osteonectin, osteopontin).
Results.
The experimental group demonstrated a significant increase in bone density (up to 2500—2700 HU vs. 1800—2200 HU in controls), a higher proportion of lamellar bone tissue (up to 24% at 5 months), and increased expression of osteogenic markers.
Conclusion.
BMP-2 coating enhances bone regeneration and improves the quality of dental implant osseointegration.

Key words:

dental implantation, BMP-2, osseointegration, bioactive coating, in vivo study

For Citation

[1]
Stepanov A.G., Apresyan S.V., Nacharyan E.G., Kopylov M.V., Levina V.S. Evaluation of the osteogenic potential of the surface of dental implants coated with recombinant BMP-2 in an in vivo experiment. Clinical Dentistry (Russia).  2026; 29 (1): 153—159. DOI: 10.37988/1811-153X_2026_1_153

References

  1. Albrektsson T., Brånemark P.I., Hansson H.A., Lindström J. Osseointegrated titanium implants. Requirements for ensuring a long-lasting, direct bone-to-implant anchorage in man. Acta Orthop Scand. 1981; 52 (2): 155—70. PMID: 7246093
  2. Adell R., Lekholm U., Rockler B., Brånemark P.I. A 15-year study of osseointegrated implants in the treatment of the edentulous jaw. Int J Oral Surg. 1981; 10 (6): 387—416. PMID: 6809663
  3. Esposito M., Hirsch J.M., Lekholm U., Thomsen P. Biological factors contributing to failures of osseointegrated oral implants. (I). Success criteria and epidemiology. Eur J Oral Sci. 1998; 106 (1): 527—51. PMID: 9527353
  4. Davies J.E. Understanding peri-implant endosseous healing. J Dent Educ. 2003; 67 (8): 932—49.
  5. Cochran D.L. A comparison of endosseous dental implant surfaces. J Periodontol. 1999; 70 (12): 1523—39. PMID: 10632528
  6. Misch C.E. Density of bone: effect on treatment plans, surgical approach, healing, and progressive boen loading. Int J Oral Implantol. 1990; 6 (2): 23—31.
  7. Javed F., Romanos G.E. Impact of diabetes mellitus and glycemic control on the osseointegration of dental implants: a systematic literature review. J Periodontol. 2009; 80 (11): 1719—30. PMID: 19905942
  8. Moy P.K., Medina D., Shetty V., Aghaloo T.L. Dental implant failure rates and associated risk factors. Int J Oral Maxillofac Implants. 2005; 20 (4): 569—77.
  9. Urist M.R. Bone: formation by autoinduction. Science. 1965; 150 (3698): 893—9. PMID: 5319761
  10. Reddi A.H. Role of morphogenetic proteins in skeletal tissue engineering and regeneration. Nat Biotechnol. 1998; 16 (3): 247—52. PMID: 9528003
  11. Wozney J.M. Overview of bone morphogenetic proteins. Spine (Phila Pa 1976). 2002; 27 (16 Suppl 1): S2—8. PMID: 12205411
  12. Wikesjo U.M., Hanisch O., Danesh-Meyer M.J., Cho K.S., Kim C.K. [Tissue engineering with rhBMP-2: Bone reconstruction in implant dentistry]. Journal of the Korean Academy of Periodontology. 1999; 29 (3): 447—468 (In Korean). DOI: 10.5051/jkape.1999.29.3.447
  13. DeConde A.S., Sidell D., Lee M., Bezouglaia O., Low K., Elashoff D., Grogan T., Tetradis S., Aghaloo T., St John M. Bone morphogenetic protein-2-impregnated biomimetic scaffolds successfully induce bone healing in a marginal mandibular defect. Laryngoscope. 2013; 123 (5): 1149—55. PMID: 23553490
  14. Zara J.N., Siu R.K., Zhang X., Shen J., Ngo R., Lee M., Li W., Chiang M., Chung J., Kwak J., Wu B.M., Ting K., Soo C. High doses of bone morphogenetic protein 2 induce structurally abnormal bone and inflammation in vivo. Tissue Eng Part A. 2011; 17 (9—10): 1389—99. PMID: 21247344
  15. Kim J.E., Lee E.J., Kim H.E., Koh Y.H., Jang J.H. The impact of immobilization of BMP-2 on PDO membrane for bone regeneration. J Biomed Mater Res A. 2012; 100 (6): 1488—93. PMID: 22396132
  16. Faensen B., Wildemann B., Hain C., Höhne J., Funke Y., Plank C., Stemberger A., Schmidmaier G. Local application of BMP-2 specific plasmids in fibrin glue does not promote implant fixation. BMC Musculoskelet Disord. 2011; 12: 163. PMID: 21762501
  17. Park J.Y., Davies J.E. Red blood cell and platelet interactions with titanium implant surfaces. Clin Oral Implants Res. 2000; 11 (6): 530—9. PMID: 11168246
  18. Carragee E.J., Hurwitz E.L., Weiner B.K. A critical review of recombinant human bone morphogenetic protein-2 trials in spinal surgery: emerging safety concerns and lessons learned. Spine J. 2011; 11 (6): 471—91. PMID: 21729796
  19. Lubelski D., Abdullah K.G., Steinmetz M.P., Alvin M.D., Nowacki A.S., Chakka S., Benzel E.C., Mroz T.E. Adverse Events With the Use of rhBMP-2 in Thoracolumbar and Lumbar Spine Fusions: A 9-Year Institutional Analysis. J Spinal Disord Tech. 2015; 28 (5): E277—83. PMID: 23429306
  20. Grigoryan A.S., Fidarov A.F. State and perspectives of studies on osteoplastic materials. Stomatology. 2016; 5: 69—74 (In Russian). DOI: 10.17116/stomat201695569-74
  21. Stepanov A.G., Apresyan S.V., Nacharyan E.G., Kopylov M.V., Kazarian G.G., Jumaniazova E.D., Karyagina V.E. Evaluation of the Cytotoxicity of BMP-2 in the Coating of Dental Implants: an In Vitro Study. Russian Journal of Dentistry. 2025; 3: 210—218 (In Russian). DOI: 10.17816/dent654080
  22. Stepanov A., Apresyan S., Gizinger O., Nacharyan E., Kopylov M. Evaluation of microbial adhesion of test cultures on the surface of structural material samples for the production of dental implants coated with bone morphogenetic protein-2. Actual Problems in Dentistry. 2025; 2: 113—117 (In Russian). DOI: 10.18481/2077-7566-2025-21-2-113-117

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Received

January 29, 2026

Accepted

February 4, 2026

Published on

March 31, 2026