DOI:

10.37988/1811-153X_2025_1_144

In vitro study of cytotoxicity and biocompatibility properties of gene-activated osteoplastic material

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Authors

  • A.I. Potapova 1, postgraduate, surgeon at the Dental surgery Division
    ORCID: 0000-0001-6651-7748
  • A.P. Vedyaeva 1, Doctor of Science in Medicine, associate professor and head of the Dental surgery Division
    ORCID: 0000-0001-7230-0799
  • T.V. Brailovskaya 1, Doctor of Science in Medicine, professor of the Surgical and experimental implantology Division
    ORCID: 0000-0003-0407-0885
  • N.V. Kosheleva 2, PhD in Biology, head of the Clinical smart-nano-technologies Lab
    ORCID: 0000-0002-2665-4972
  • P.S. Timashev 2, Doctor of Science in Chemistry, associate professor and head of the Biomedical Science & Technology Park
    ORCID: 0000-0001-7773-2435
  • I.I. Vlasova 2, PhD in Physics and Mathematics, leading researcher of the division of Modern biomaterials
    ORCID: 0000-0003-1813-654X
  • P.I. Koteneva 2, junior researcher at the “Biofactory” Design Centre
    ORCID: 0000-0001-9428-8487
  • A.M. Tsitsiashvili 3, Doctor of Science in Medicine, professor of the Oral surgery propaedeutics Department
  • 1 Central Research Institute of Dental and Maxillofacial Surgery, 119021, Moscow, Russia
  • 2 Sechenov University, 127006, Moscow, Russia
  • 3 Russian University of Medicine, 127006, Moscow, Russia

Abstract

A study of the biocompatibility and cytotoxicity of two osteoplastic materials has been conducted: the xenogenic material (XM) “Bio-Oss” and the gene-activated material (GAM) “Nucleostim-VEGF”. The experiment was conducted using blood, which was incubated with samples of materials at 37°C. Chemiluminescent reactions of neutrophils under the influence of the activator forbol-12-myristate-13-acetate, as well as the adhesion of multipotent mesenchymal stromal cells (MMSCs) to materials were studied. The results showed that both materials enhance the radical-generating activity of neutrophils, but at different rates: GAM activates cells faster, reaching a maximum after 40 minutes, while XM takes a longer time to activate. MMSCs successfully adhered to the granules of both materials, but GAM provided better cell viability. The data obtained indicate the high biocompatibility of GAM and its potential for use in tissue engineering and regenerative medicine.

Key words:

plasmid, osteoplastic material, gene-activated material, macrophages, multipotent mesenchymal stromal cells, adhesion

For Citation

[1]
Potapova A.I., Vedyaeva A.P., Brailovskaya T.V., Kosheleva N.V., Timashev P.S., Vlasova I.I., Koteneva P.I., Tsitsiashvili A.M. In vitro study of cytotoxicity and biocompatibility properties of gene-activated osteoplastic material. Clinical Dentistry (Russia).  2025; 28 (1): 144—151. DOI: 10.37988/1811-153X_2025_1_144

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Received

September 6, 2024

Accepted

March 1, 2025

Published on

April 7, 2025